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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels <t>of</t> <t>IL-17C,</t> IL-20 and of IL-8 and IL-6 were measured by <t>ELISA</t> and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.
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Image Search Results


Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels of IL-17C, IL-20 and of IL-8 and IL-6 were measured by ELISA and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.

Journal: Journal of dermatological science

Article Title: Calcipotriol and betamethasone dipropionate exert additive inhibitory effects on the cytokine expression of inflammatory dendritic cell-Th17 cell axis in psoriasis.

doi: 10.1016/j.jdermsci.2015.12.009

Figure Lengend Snippet: Fig. 5. Treatment with calcipotriol and betamethasone dipropionate impairs secretion of pro-inflammatory cytokines by human KCs. Human KC monolayer cultures were treated with calcipotriol (100 nM), betamethasone (1 mM), calcipotriol in combination with betamethasone (100 nM/1 mM; ratio 1:10) followed by stimulation with the mix of IL-17A, IL-22 and TNF-a for 48 h. The levels of IL-17C, IL-20 and of IL-8 and IL-6 were measured by ELISA and MSD, respectively. The results expressed as the percentage difference compared to vehicle treatment control are mean values SEM from independent experiments using human primary KCs from three (n = 3) different donors. One-way ANOVA followed by Tukey’s multiple comparison tests were used to compare treatment effect. ****P 0.0001; ***P 0.001; **P 0.01.

Article Snippet: After 48 h of treatment, cell culture supernatants were collected for protein analysis of IL-20 (Human IL-20 Quantikine) and IL-17C (Human IL-17C DuoSet) using ELISA kits from R&D Systems and of IL-8 and IL-6 using Human ProInflammatory-4 II Ultra-Sensitive Kit from Meso Scale Diagnostics.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison